CEACAM1 Mouse Monoclonal Antibody [Clone ID: B3]

Specifications

Product Data
Clone Name	B3
Applications	ELISA, FC, IF, IHC, IP, WB
Recommend Dilution	ELISA: Use at 2-5 µg/ml. Western blot: Use at 1-5 µg/ml. Flow Cytometry: Use at 10 µg/ml. Immunofluorescence/Immunohistochemistry: Use at 2-5 µg/ml. Immunohistochemistry on Paraffin Sections. Immunoprecipitation: Use at 10 µg/IP. Affinity purification.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Human soluble CEACAM-1-Fc lacking the N-domain (produced in HEK293 cells)
Specificity	This Monoclonal CEACAM-1 antibody (B3) interacts solely with Human CEACAM-1 and shows no cross-reactivity with other CEACAMs expressed in Human or other species! B3 binds to the A1-B-Domain! Does not react with Rat, Mouse, Dog and Cattle.
Formulation	PBS, pH 7.4 State: Purified State: Lyophilized purified IgG fraction of Cell Culture Supernatant
Reconstitution Method	Restore in sterile water corresponding to a concentration of 0.1-1.0 mg/ml. Centrifuge vial prior to opening.
Purification	Protein G Chromatography
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	carcinoembryonic antigen related cell adhesion molecule 1
Database Link	Entrez Gene 634 Human UniProt ID P13688
Background	Carcinoembryonic antigen (CEA)-related cell adhesion molecule 1 (CEACAM1? also BGP) is a 160 kDa member of the CEACAM branch of the CEA gene family of the immunoglobulin superfamily (1-3). It is one of seven human CEACAM subfamily genes that are essentially divided equally between type I trans-membrane proteins (CEACAM1, 3-4) and GPI-linked molecules (CEACAM5-8). There is no CEACAM2 in human. The gene for human CEACAM1 codes for a 526 amino acid (aa) type I transmembrane protein that contains a 34 aa signal sequence, a 394 aa extracellular domain (ECD), a 24 aa transmembrane segment, and a 74aa cytoplasmic region (4, 5). The ECD contains one N-terminal V-type followed by three C2-type Ig-like domains. It shows considerable glycosylation (1). There are three soluble and seven transmembrane isoforms. The three soluble forms also contain the first two C2-type Ig like domains (aa’s 145 317), with differences coming in the third C2-type Ig- like domain (6). The seven transmembrane isoforms are highly divergent. Full-length mouse and rat CEACAM1 are approximately 57% aa identical to human CEACAM1? in the V-type Ig-like domain, they are 58% and 56% aa identical, respectively. The full-length molecule is found on neutrophils, bile duct epithelium, activated NK cells, colonic columnar epithelium and endothelium.
Synonyms	BGP, BGP1, Biliary glycoprotein 1
Reference Data
Protein Families	Druggable Genome, Transmembrane

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