beta Catenin (CTNNB1) Mouse Monoclonal Antibody [Clone ID: EM-22]

Specifications

Product Data
Clone Name	EM-22
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	Flow Cytometry. Immunoprecipitation. Western blot. Positive Control: HT29 Human colon adenocarcinoma cell line, FHC human cell line, DLD1 human colon adenocarcinoma cell line, KW1 mouse cell line C57MG mouse cell line, 3T3 mouse fibroblast cell line. Immunocytochemistry. Positive Control: HT29 human colon adenocarcinoma cell line. This clone has been described to work in Immunohistochemitry on Paraffin Sections.
Reactivity	Hamster, Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Human beta-Catenin
Specificity	This antibody reacts with C-terminal part of beta-Catenin, an 88 kDa multifunctional protein involved both in cell adhesion and in activation of transcription.
Formulation	PBS, pH~7.4 State: Aff - Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE) Preservative: 15 mM Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	catenin beta 1
Database Link	Entrez Gene 12387 MouseEntrez Gene 1499 Human UniProt ID P35222
Background	Beta-catenin is a multifunctional protein involved both in cell adhesion and in activation of transcription. Calcium-dependent intercellular adhesion transmembrane glycoprotein E-cadherin interacts by its cytoplasmic domain with reciprocally bound alpha, beta and gamma catenin. Beta-catenin links this complex through alpha-actinin to the cytoskeleton. Functional cadherin-catenin system is important for invasiveness of tumour cells. Beta-catenin level in cytoplasm is controlled by glycogen synthase kinase-3 beta. When activity of this kinase is blocked (e.g. by excessive stimulation of Wnt signaling pathway), hypophosphorylated stable form of beta-catenin accumulates in the cytoplasm, translocates to the nucleus and activates transcription of genes including those that are involved in cell cycle control. As a result, cell division and neoplastic transformation are promoted.
Synonyms	CTNNB1, CTNNB, Beta-catenin
Reference Data
Protein Families	Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors
Protein Pathways	Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Basal cell carcinoma, Colorectal cancer, Endometrial cancer, Focal adhesion, Leukocyte transendothelial migration, Melanogenesis, Pathogenic Escherichia coli infection, Pathways in cancer, Prostate cancer, Thyroid cancer, Tight junction, Wnt signaling pathway

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