Pecam1 Rat Monoclonal Antibody [Clone ID: MEC7.46]

Specifications

Product Data
Clone Name	MEC7.46
Applications	IF, IHC, IP
Recommend Dilution	Immunohistochemistry on Frozen Sections (1,3): Tissue sections were fixed in acetone for 10 min at RT and pretreated with 0.01% Hydrogen peroxide to quench endogenous peroxidases. Normal rabbit serum was used as blocking agent. As (Ref.1). The typical starting working dilution is 1:50. Flow Cytometry (1): Stains the extracellular domain of mouse PECAM-1; 106 cells were used per sample. No fixation or permeabilization needed. Epitope is sensitive to trypsin! (Ref.1). The typical starting working dilution is 1:50. Immunocytochemistry (1,2). Immunoprecipitation (1): Lysate of 35S-Methionine labeled 5HV cells was used. Molecule of ~130 kDa. Positive Control: Mouse t-end.1 cells. Negative Control: L929 cells (Fibroblasts).
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Mouse t.end.1 cells (polyoma middle T (PmT)-transformed EC)
Specificity	The monoclonal antibody MEC7.46 recognizes the mouse form of the platelet-endothelial cell adhesion molecule (PECAM)-1 (CD31). The reactivity of the monoclonal antibody MEC7.46 is restricted to the isoform of the molecule that is selectively expressed by endothelial cells. The antibody precipitates a 130 kDa molecule present on the membrane of endothelial cells of all mouse blood vessels both in normal, inflamed and tumor tissues. The antigen is predominantly present at the lateral borders of endothelial cells as described for human PECAM-1. Staining of MEC7.46 can be seen on capillaries, veins, arteries and liver sinusoids.
Formulation	PBS State: Purified State: Liquid 0.2 µm filtered Ig fraction Stabilizer: 0.1% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Protein G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Gene Name	platelet/endothelial cell adhesion molecule 1
Database Link	Entrez Gene 18613 Mouse UniProt ID Q08481
Background	PECAM-1 is a member of the immunoglobulin superfamily. This heavily glycosylated protein is found in the entire vascular endothelium of adult mice and functions in mediating cellular adhesion by heterophilic and homophilic mechanisms. PECAM-1 is detected within the lymphopoietic islands in the spleen of newborn (day 12) and in the bone marrow of adult mice. Capillary endothelial cells of adult mice also express PECAM-1.
Synonyms	PECAM-1, EndoCAM, GPIIA'
Reference Data

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