CD5 Mouse Monoclonal Antibody [Clone ID: L17F12]

CAT#: AM26700PU-N

CD5 mouse monoclonal antibody, clone L17F12, Purified

Conjugation: Unconjugated APC Biotin FITC PE



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CNY 3,140.00


货期*
5周

规格
    • 100 ug

Product images

Specifications

Product Data
Clone Name L17F12
Applications FC, IF, IP, WB
Recommend Dilution Flow cytometry: 1-4 μg/ml.
Immunoprecipitation.
Western blot: Laurylmaltoside lysing buffer; non-reducing conditions; 1-2 μg/ml.
Immunocytochemistry.
Reactivity Human
Host Mouse
Clonality Monoclonal
Immunogen Human acute lymphoblastic leukemia (ALL) T cells
Specificity The antibody reacts with CD5, a 67kDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most of thymocytes and B lymphocytes subset (B-1a lymphocytes).
Formulation Phosphate buffered saline (PBS), approx. pH 7.4
State: Purified
State: Liquid Ig fraction
Preservative: 15 mM sodium azide
Concentration lot specific
Purification Protein-A affinity chromatography (> 95% pure by SDS-PAGE)
Conjugation Unconjugated
Storage Condition Store undiluted at 2-8°C.
DO NOT FREEZE!
Gene Name CD5 molecule
Background

CD5 antigen (T1; 67 kDa) is a human cell surface T-lymphocyte single-chain transmembrane glycoprotein. CD5 is expressed on all mature T-lymphocytes, most of thymocytes, subset of B-lymphocytes and on many T-cell leukemias and lymphomas. It is a type I membrane glycoprotein whose extracellular region contains three scavenger receptor cysteine-rich (SRCR) domains.
The CD5 is a signal transducing molecule whose cytoplasmic tail is devoid of any intrinsic catalytic activity. CD5 modulates signaling through the antigen-specific receptor complex (TCR and BCR). CD5 crosslinking induces extracellular Ca++ mobilization, tyrosine phosphorylation of intracellular proteins and DAG production. Preliminary evidence shows protein associations with ZAP-70, p56lck, p59fyn, PC-PLC, etc. CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and development stage. In thymocytes and B1a cells seems to provide inhibitory signals, in peripheral mature T lymhocytes it acts as a costimulatory signal receptor. CD5 is the phenotypic marker of a B cell subpopulation involved in the production of autoreactive antibodies.
Disease relevance: CD5 is a phenotypic marker for some B cell lymphoproliferative disorders (B-CLL, Hairy cell leukemia, etc.). The CD5+ popuation is expanded in some autoimmune disorders (Rheumatoid Arthritis, etc.). Herpes virus infections induce loss of CD5 expression in the expanded CD8+ human T cells.

Synonyms CD5, LEU1
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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