Interferon gamma (IFNG) Mouse Monoclonal Antibody [Clone ID: 4S.B3]
CAT#: AM26712PU-N
Interferon gamma (IFNG) mouse monoclonal antibody, clone 4S.B3, Purified
Conjugation: APC Biotin FITC PE
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CNY 3,140.00
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Specifications
Product Data | |
Clone Name | 4S.B3 |
Applications | ELISA, FC, FN, IF, IHC, IP, R, WB |
Recommend Dilution | Flow Cytometry: Intracellular staining.
Immunoprecipitation.
Western Blot.
Immunohistochemistry on Paraffin Sections.
Immunocytochemistry.
ELISA: Used as detection antibody in combination with capture antibody NIB42.
RIA.
Functional Application: Neutralization (weak). |
Reactivity | Human, Primate |
Host | Mouse |
Clonality | Monoclonal |
Immunogen | Interferon gamma derived from Human leukocytes |
Specificity | This antibody 4S.B3 recognizes IFN-gamma, a 16-25 kDa cytokine produced by activated Th1 cells and NK cells. Binds both glycosylated and non-glycosylated protein. |
Formulation | PBS, pH~7.4 State: Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE) Preservative: 15 mM Sodium Azide |
Concentration | lot specific |
Purification | Affinity Chromatography on Protein A |
Conjugation | Unconjugated |
Storage Condition | Store undiluted at 2-8°C. DO NOT FREEZE! |
Gene Name | interferon, gamma |
Database Link | |
Background | The Interferon gamma (IFN-gamma; 16-25 kDa) is an important regulator of the immune response, produced in activated Th1 cells and NK cells, particularly in response to IL-2, TNF-alpha and IL-12; its production is suppressed by IL-4, IL-10, and TGF-beta. The producing of IFN-gamma is activated by specific antigens or mitogens through the T cell antigen receptor. IFN-gamma polypeptide forms: 40-60 kDa forms are observable under non-denaturing conditions as dimers and trimers; 20 kDa and 25 kDa forms exist due to variable glycosylation. IFN-gamma belongs to the type II interferons, also called immune IFN. IFN-gamma shows antiviral activity and has important immunoregulatory functions. It is a potent activator of macrophages and had antiproliferative effects on transformed cells. IFN-gamma plays an important role in regulating B cell differentiation by simultaneously stimulating class switch recombination to the IgG3 and IgG2a isotypes while represing class switch recombination to the IgE and IgG1 isotypes. It also appears to promote antigen presentation by B cells through its effects on MHC. Binding of IFN-gamma to its receptor increases the expression of class I MHC on all somatic cells. It also enhances the expression of class II MHC on antigen-presenting cells. IFN-gamma is the major means by which T cells activate macrophages, increasing their ability to kill bacteria, parasites, and tumours. The activation of macrophages by IFN-gamma is essential for the elimination of bacteria that replicate within the phagosomes of macrophages (f.e. Mycobacteria and Listeria monocytogenes). IFN-gamma can potentiate the high antiviral and antitumor effects of the type I interferons (IFN-alpha, IFN-beta). IFN-gamma may also activate neutrophils and NK cells. |
Synonyms | IFN-gamma, gamma IFN |
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