MRP8 (S100A8) Mouse Monoclonal Antibody [Clone ID: CF-145]
CAT#: AM31838FC-N
MRP8 (S100A8) mouse monoclonal antibody, clone CF-145, FITC
Conjugation: Unconjugated Biotin
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CNY 4,829.00
Specifications
Product Data | |
Clone Name | CF-145 |
Applications | FC, IF, IHC, WB |
Recommend Dilution | Flow Cytometry: 0.5 µg/1x106 cells. Immunofluorescence. Western blot. Immunohistochemistry on Paraffin Sections: 1/50. Heat induced antigen retrieval in pH 6.0 citrate buffer is recommended. |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Immunogen | Purified granulocyte antigen of Human origin. Donor: Human leukocytes. Fusion Partner: NSO Mouse Myeloma Partner. |
Specificity | This antibody detects Calgranulin A (S100A8). |
Formulation | PBS Label: FITC State: Liquid purified IgG fraction of Ascitic fluid Stabilizer: EIA grade BSA to bring total protein concentration to 4-5 mg/ml Preservative: 0.02% Sodium Azide |
Concentration | lot specific |
Purification | Protein G Chromatography |
Conjugation | FITC |
Storage Condition | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing. |
Gene Name | S100 calcium binding protein A8 |
Database Link | |
Background | Calgranulin A, also known as MRP-8 and S100A8 is a member of the S100 family of proteins containing 2 EF hand (alpha helix, turn, alpha helix structure) calcium binding motifs. S100 proteins are localized in the cytoplasm and /or nucleus of a wide range of cells and are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. MRP-8 (S100A8) forms a heterodimeric complex with Calgranulin B, (MRP-14, S100A9) in the cytosol of monocyte and neutrophil cell types circulating in peripheral blood. Calgranulin A is found in elevated levels in the serum of cystic fibrosis cases and is also expressed in the skin of patients with psoriasis, eczematous dermatitis and squamous cell carcinoma. |
Synonyms | S100-A8, CAGA, MRP-8, CFAG |
Note | Protocol: Tests Results Tissue Distribution by Flow Cytometry Analysis: Cell Concentration : 1x10e6 cells per tests. Antibody Concentration Used: 0.5 μg/10e6 cells. Isotypic Control (shaded): FITC Mouse IgG2b. |
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