PF4 Mouse Monoclonal Antibody [Clone ID: KKO]

Specifications

Product Data
Clone Name	KKO
Applications	ELISA, WB
Recommend Dilution	ELISA: Use at 0.08 μg/ml following coating plate with PF4/ heparin complex. This can be done by incubating PF4 with an excess of heparin (100-200 U/ml), while coating the plate. Western blot.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human Factor IV purified from the supernatant of Thrombin activated platelets.
Specificity	This antibody is specific for Human Platelet Factor IV/heparin complexes. AM31851BT-N (clone KKO), unlike AM31850BT-N (clone RTO) which binds PF4 alone, is specific for PF4/heparin complexes and is seen to have similar binding properties as auto-antibodies found in HIT/HITT.
Formulation	PBS containing 0.02% Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml Label: Biotin State: Liquid purified IgG fraction
Concentration	lot specific
Conjugation	Biotin
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	platelet factor 4
Database Link	Entrez Gene 5196 Human UniProt ID P02776
Background	Platelet Factor IV (PF4) is a 70 aa protein released from the alpha granules of activated platelets. PF4 is synthesized by bone marrow megakaryocytes and stored in alpha granules as a non-covalent bound tetramer. Platelet factor IV binds with high affinity to heparin and plays a role in inflammation and wound repair. PF4 is a chemoattractant for neutrophils, monocytes and fibroblasts and has been reported to be an immunologic regulator that inhibits suppressor T-cell activity. Heparin-Induced Thrombocytopenia/Thrombosis (HIT/HITT) is a life-threatening complication that manifests itself in a small population of patients exposed to intravenous-heparin. It is characterized by the production of PF4/heparin auto-antibodies. These auto-antibodies bind the PF4/heparin complexes and subsequently bind the FCGIIa receptor on the platelets surface through their Fc region. This activates the platelets and can initiate clot formation.
Synonyms	PF-4, Iroplact, Oncostatin-A, CXCL4, SCYB4
Reference Data
Protein Families	Druggable Genome, Secreted Protein, Transmembrane
Protein Pathways	Chemokine signaling pathway, Cytokine-cytokine receptor interaction

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