MRP6 (ABCC6) Rat Monoclonal Antibody [Clone ID: M6II-31]

Specifications

Product Data
Clone Name	M6II-31
Applications	FC, IF, IHC, WB
Recommend Dilution	Western blotting: Use 1/20-1/50 dilution and HRP-anti-Rat-IgG. Flow Cytometry (optimal conditions still to be defined). Immunocytochemistry: Use 1/20-1/50 on Acetone Fixed Cytospin preparations. Immunohistochemistry on Frozen Sections: 1/20 on Acetone Fixed Frozen Sections can be followed by incubation with Rabbit anti-Rat IgG (1/25) and an APAAP complex (1/50). Immunohistochemistry on Paraffin Sections: M6II-31 can be used on Formaldehyde-Fixed Paraffin-Embedded Human tissues, after pretreatment with 0.01 M citric acid (pH 6.0) in distilled water at 100°C for 10 minutes. After incubation of M6II-31 (use 1/20) and washing, slides can be incubated with Biotinylated Rabbit anti-Rat IgG (1/100) and streptavidin conjugated to Horseradish Peroxidase (1/500). M6II-31 can be used to detect Human MRP6 in cells and tissues.
Reactivity	Human
Host	Rat
Clonality	Monoclonal
Immunogen	Bacterial fusion protein of Human MRP6, containing amino acids 764-964, spanning the putative 12th transmembrane region as well as predicted internal and external regions of the protein.
Specificity	This Monoclonal antibody clone M6II-31 reacts with MRP6, a 190-200 kD transmembrane protein that is related to the multidrug resistance related protein MRP. M6II-7 did not cross-react with the Human MDR1, MRP1, MRP2, MRP3, MRP4, MRP5 gene products.
Formulation	State: Supernatant State: Liquid Tissue Culture Supernatant (Serum Free) Stabilizer: 0.7% BSA Preservative: 0.09% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	ATP binding cassette subfamily C member 6
Database Link	Entrez Gene 368 Human UniProt ID O95255
Background	The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. Defects in ABCC6 are the cause of pseudoxanthoma elasticum (PXE) [MIM:264800]. PXE is a disorder characterized by calcification of elastic fibers in skin, arteries and retina that results in dermal lesions with associated laxity and loss of elasticity, arterial insufficiency and retinal hemorrhages leading to macular degeneration. PXE is caused in the overwhelming majority of cases by homozygous or compound heterozygous mutations in the ABCC6 gene (autosomal recessive PXE). Individuals carrying heterozygous mutations express limited manifestations of the pseudoxanthoma elasticum phenotype (autosomal dominant PXE).
Synonyms	Multidrug resistance-associated protein 6, ARA, MOAT-E
Note	MAb producing cells: The hybridoma cell line was obtained by fusion of lymph node cells from an immunized rat (Wistar) with SP2/O mouse myeloma cells. Culture Medium: RPMI-1640 (Gibco, Paisley, Scotland UK), supplemented with Nutridoma-SR (Boehringer, Indianapolis, USA). The medium does not contain serum nor added enzymes. The antibody solution has been filtered through a 0.22 micron filter. NOTE: This monoclonal antibody has been produced in a clinical laboratory in which no animal viruses are being studied or cultured.
Reference Data

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