Major Basic Protein (PRG2) Mouse Monoclonal Antibody [Clone ID: BMK-13]

Specifications

Product Data
Clone Name	BMK-13
Applications	ELISA, IHC
Recommend Dilution	ELISA.  Immunohistochemistry on frozen and paraffin sections: It stains in bronchial sections 98% of the periphere blood eosinophils. Most useful for immunohistochemistry on frozen sections is the APAAP method. BMK-13 also binds to eosinophils in paraffin-embedded tissue. A 1:30 dilution in PBS is recommended. Incubation time: 1 hour at RT. Enzymatic pretreatment is necessary. We recommend to use pepsine. Do NOT use heat denaturation. This antibody is suitable for blood smears (cytospins) if fixed with methanol-acetone mixture (1/1, v/v).  It is unlikely, without permeabilization, that the BMK13 is suitable for the flow cytometry because BMK13 stains intracellular, stored proteins.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Specificity	BMK-13 binds to the 10 kD eosinophil Major Basic Protein (MBP) of both resting and activated eosinophils in cytospins and frozen sections of bronchial and skin biopsies of allergic sites and normal sites and thus can be used as a "pan-eosinophil" marker. The antibody BMK-13 stains in frozen sections of bronchial biopsies from atopic asthmatics, rhinitics and normal non-atopic subjects, substantially higher counts of positive cells when compared to EG1, EG2 and chromotrope 2R. BMK-13 cross-reacts weakly with human basophils, which also contain low level of this protein. It does not cross-react with any other human protein or cell.
Formulation	PBS State: Purified State: Liquid purified Ig fraction Stabilizer: 0,1% BSA Preservative: 0,02% sodium azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	proteoglycan 2, bone marrow (natural killer cell activator, eosinophil granule major basic protein)
Database Link	Entrez Gene 5553 Human UniProt ID P13727
Synonyms	PRG2, Bone marrow proteoglycan, BMPG, EMBP
Reference Data

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