TAG72 Mouse Monoclonal Antibody [Clone ID: B72.3 + CA72/733]

CAT#: AM33341PU-S

TAG72 mouse monoclonal antibody, clone B72.3 + CA72/733, Purified

Size: 20 ug 100 ug



Need it in bulk or conjugated?
Get a free quote

CNY 5,606.00


货期*
5周

规格
    • 100 ug

Product images

Specifications

Product Data
Clone Name B72.3 + CA72/733
Applications FC, IF, IHC, IP, WB
Recommend Dilution Western Blot: 0.5-1 µg/ml. 
Flow Cytometry: 0.5-1 µg/106 cells. 
Immunoprecipitation: 1-2 µg/500 µg protein lysate. 
Immunofluorescence: 1-2 µg/ml.
Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT.
No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues. 
Positive Control: Jurkat cells, Breast or lung carcinoma.
Reactivity Bovine, Canine, Hamster, Human, Rat
Host Mouse
Clonality Monoclonal
Immunogen TAG-72 protein (B72.3 & CA72/733).
Specificity This antibody recognizes an oncofetal antigen of 220kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin.
This Monoclonal antibody defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80% sensitivity and 93% specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of anti-TAG-72 and anti-GCDFP-15 is valuable in the diagnosis of apocrine carcinoma.
Cellular Localization: Cell surface and cytoplasmic.
Formulation 10mM PBS
State: Purified
State: Liquid purified IgG fraction from Bioreactor Concentrate
Stabilizer: 0.05% BSA
Preservative: 0.05% Sodium Azide
Concentration lot specific
Purification Protein A/G Chromatography
Conjugation Unconjugated
Storage Condition Store undiluted at 2-8°C.
DO NOT FREEZE!
Predicted Protein Size 220 kDa
Background Tumor-associated glycoprotein (TAG) 72 is a tumor marker, identified and characterized using two different monoclonal antibodies B72.3 and CC49. It is a mucin-like protein of high molecular weight (220-400 kd). The protein is expressed in several epithelial-derived cancers including 94% of colonic adenocarcinomas, 84% of invasive ductal carcinomas of the breast, 96% of non-small cell lung carcinomas, 100%of common epithelial ovarian carcinomas, as well as the majority of pancreatic, gastric, and esophageal cancers evaluated. The protein expression is also detected in fetal colon, stomach, and esophagus, thus defining TAG-72 as an oncofetal antigen.
Synonyms CA 72-4, CA72-4, sialy-Tn, Tumor-Associated Glycoprotein 72
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
Customer Reviews 
Loading...