c-Myc (MYC) Mouse Monoclonal Antibody [Clone ID: MYC699]

Specifications

Product Data
Clone Name	MYC699
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	ELISA: Use BSA free Antibody for coating. Flow Cytometry: 0.5-1 μg/million cells. Immunofluorescence: 1-2 μg/ml. Western Blotting: 0.5-1 μg/ml. Immunoprecipitation: 1-2 μg/500 μg protein lysate. Immunohistochemistry on Frozen Sections: 0.5-1.0 μg/ml for 30 minutes at RT. Positive Control: HL-60 cells or breast carcinoma.
Reactivity	Chimpanzee, Human, Gorilla
Host	Mouse
Clonality	Monoclonal
Immunogen	A synthetic peptide, corresponding to aa 408-439 (AEEQKLISEEDLLRKRREQLKHKLEQL-RNSCA) from C-terminus of Human c-myc, coupled to KLH.
Specificity	Recognizes c-Myc Oncoprotein. Cellular Localization: Nuclear.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	62-64 kDa
Gene Name	v-myc avian myelocytomatosis viral oncogene homolog
Database Link	Entrez Gene 4609 Human UniProt ID P01106
Background	The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiatio n, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/ Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase ( JNK) in cell proliferation and cell cycle regulation. Studies also have shown that c -Myc is essential for tumor cell development in vasculogenesis and angiogenesis that distribut e blood throughout the cells, and which brought extensive attention in the development of new therapeutic approach for cancer treatment.
Synonyms	Transcription factor p64, BHLHE39
Reference Data

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