Myeloperoxidase (MPO) Mouse Monoclonal Antibody [Clone ID: 266.6K2]

Specifications

Product Data
Clone Name	266.6K2
Applications	FC, IF
Recommend Dilution	Clone 266.6K2 can be applied in Flow Cytometry, or in immunohistochemistry using cytospots or frozen tissue sections. The combination of anti-MPO with a fixation / permeabilization reagent for peripheral blood leukocytes is recommended for the determination of intracellular MPO by Flow Cytometry.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Specificity	Clone 266.6K2 reacts with the intracellular enzyme MPO. In a study comparing anti-MPO with other myeloid-reactive monoclonal antibodies such as anti-CD13, CD14, CD15 and CD33, a higher diagnostic sensitivity and specificity was found for AML using anti-MPO. In most patients, more MPO-positive cells were detected by anti-MPO than by cytochemical staining with Sudan black and standard peroxidase. Testing by Flow Cytometry using a fixation and permeabilization solution on whole blood from healthy donors showed the following values expressed in terms of % of the total leukocyte count: Product code: AM39035FC-N (anti-MPO FITC).  n: 10 Mean % positive: 64,06 S.D.: 5,07 % CV: 7,92
Formulation	0.01 M sodium phosphate, 0.15 M NaCl, pH 7.3, 0.2% BSA, 0.09% sodium azide Label: FITC State: Liquid purified IgG fraction Label: Cat. No. Label EX-max (nm) / EM-max (nm): AM39035FC-N 488 / 519 AM39035PU-N Pure . /
Concentration	lot specific
Purification	Affinity chromatography
Conjugation	FITC
Storage Condition	Store the antibody undiluted at 2-8°C. Fluorochrome labelled product is photosensitive and should be protected from light.
Gene Name	myeloperoxidase
Database Link	Entrez Gene 4353 Human UniProt ID P05164
Background	Myeloperoxidase (MPO) is an intracellular enzyme in peripheral blood granulocytes. Anti-MPO antibodies stain promyelocytes, myelocytes, metamyelocytes and mature neutrophil granulocytes in bone marrow. Monocytes are negative or slightly positive while eosinophils and lymphocytes are negative. Anti-MPO is used for the diagnosis of AML (acute myeloid leukemia). Non-myeloid leukemia (e.g. acute lymphoblastic leukemia) is usually non-reactive with anti-MPO.
Synonyms	MPO
Note	1. Conjugates with brighter fluorochromes, like PE and APC, will have a greater separation than those with dyes like FITC. When populations overlap, the percentage of positive cells using a selected marker can be affected by the choice of fluorescent label. 2. Use of monoclonal antibodies in patient treatment can interfere with antigen target recognition by this reagent. This should be taken into account when samples are analyzed from patients treated in this fashion. 3. Reagent data performance is based on EDTA-treated blood. Reagent performance can be affected by the use of other anticoagulants.
Reference Data
Protein Families	Druggable Genome

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