HSP27 (HSPB1) Mouse Monoclonal Antibody [Clone ID: SPM252]

Specifications

Product Data
Clone Name	SPM252
Applications	IF, IHC, IP, WB
Recommend Dilution	ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: ~50% of Breast Carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor.
Reactivity	Chimpanzee, Chicken, Human, Mouse, Rat, Sheep
Host	Mouse
Clonality	Monoclonal
Immunogen	Partially purified hsp27 (earlier called 24K) protein from breast cancer MCF-7 cells.
Specificity	It recognizes a 24-27kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). Hsp27 was recently found to be identical to the estrogen-induced “p29” and “24K” protein. About 50% of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells. Cellular Localization: Predominantly cytoplasmic with some nuclear.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	27 kDa
Gene Name	heat shock protein family B (small) member 1
Database Link	Entrez Gene 3315 Human UniProt ID P04792
Synonyms	Heat shock protein beta-1, Heat shock 27 kDa protein, HSP28, 28 kDa heat shock protein, SRP27, HSP25
Reference Data

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