Involucrin (IVL) Mouse Monoclonal Antibody [Clone ID: SPM259]

Specifications

Product Data
Clone Name	SPM259
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 1-2 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 1-2 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 1-2 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues is ENHANCED by digestion with trypsin (or Protease XXV) at 1mg/ml PBS for 5 min at 37°C). Positive Control: MCF-7 cells. Localized to upper spinous and granular layers in normal skin.
Reactivity	Canine, Human, Monkey, Porcine, Gorilla
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified involucrin from Human keratinocytes.
Specificity	It recognizes a protein of 66kDa-170kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120kDa protein. It stains the involucrin in a variety of sizes: 170kDa in MCF-7 cells, a doublet of ~115kDa and 150kDa in gorilla and owl monkey, 66kDa in dog, and a doublet of 105kDa in pig. Its epitope maps between codon 421-568 of human involucrin. Cellular Localization: Cytoplasmic.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	66-170 kDa
Gene Name	involucrin
Database Link	Entrez Gene 3713 Human UniProt ID P07476
Background	Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal; expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.
Synonyms	involucrin
Reference Data

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