Cytokeratin 18 (KRT18) Mouse Monoclonal Antibody [Clone ID: SPM510]

CAT#: AM50133PU-S

Cytokeratin 18 (KRT18) mouse monoclonal antibody, clone SPM510, Purified

Size: 20 ug 100 ug



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CNY 5,830.00


货期*
5周

规格
    • 100 ug

Product images

Specifications

Product Data
Clone Name SPM510
Applications FC, IF, IHC, IP, WB
Recommend Dilution Flow Cytometry: 0.5-1 μg/million cells.
Immunofluorescence: 1-2 μg/ml.
Western Blotting: 0.5-1 μg/ml.
Immunoprecipitation: 1-2 μg/500 μg protein lysate.
Immunohistochemistry on Formalin-fixed Sections: 0.5-1.0 μg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Recommended Positive Control: MCF-7, HeLa cells, Breast Cancer.
Reactivity Human
Host Mouse
Clonality Monoclonal
Immunogen Human breast cancer PMC 42 cells.
Specificity This Monoclonal Antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
Cellular Localization: Cytoplasmic.
Negative Species: Mouse, Rat, Hamster, Cow, Dog, Pig, and Sheep.
Formulation 10mM PBS
State: Purified
State: Liquid purified IgG fraction from Bioreactor Concentrate
Stabilizer: 0.05% BSA
Preservative: 0.05% Sodium Azide
Concentration lot specific
Purification Protein A/G Chromatography
Conjugation Unconjugated
Storage Condition Store undiluted at 2-8°C.
Predicted Protein Size 45 kDa
Gene Name keratin 18
Synonyms Cytokeratin-18, CK18, Keratin-18, Keratin 18, KRT18, CYK18, K18
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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