EMA (MUC1) Mouse Monoclonal Antibody [Clone ID: SPM132]

Specifications

Product Data
Clone Name	SPM132
Applications	FC, IF, IHC
Recommend Dilution	Flow Cytometry: 0.5-1 μg/million cells. Immunofluorescence: 1-2 μg/ml. Immunohistochemistry on Formalin-Fixed Paraffin Sections: 0.5-1.0 μg/ml for 30 minutes at RT. No special pretreatment is required for staining of formalin/paraffin tissues. Positive Control: MCF-7 or MDA-231 cells. Breast or colon carcinoma.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human milk fat globule membranes.
Specificity	This Monoclonal Antibody reacts with the DTRP epitope in the tandem repeats. The alpha subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling, through phosphorylations and protein-protein interactions. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinoma tissues. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver. Cellular Localization: Cytoplasmic and cell surface.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	265-400 kDa
Gene Name	mucin 1, cell surface associated
Database Link	Entrez Gene 4582 Human UniProt ID P15941
Synonyms	MUC-1, PEMT, Episialin, EMA, H23AG, PUM, DF3, CA 15-3
Reference Data

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