Mucin 5AC (MUC5AC) Mouse Monoclonal Antibody [Clone ID: SPM297]

Specifications

Product Data
Clone Name	SPM297
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	ELISA: Use BSA free Antibody for coating. Flow Cytometry: 0.5-1 μg/million cells. Immunofluorescence: 1-2 μg/ml. Western Blotting: 0.5-1 μg/ml. Immunoprecipitation: 1-2 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-fixed Sections: 0.5-1.0 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: Stomach.
Reactivity	Chicken, Feline, Human, Monkey, Mouse, Porcine, Rabbit, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient.
Specificity	This MAb recognizes the peptide core of gastric mucin M1 (>1,000kDa) (recently identified as Mucin 5AC). Its epitope is destroyed by beta-mercaptoethanol and proteases but not by periodate treatment. Antibody to gastric mucin M1 reacts with the gastric epithelium of normal human gastrointestinal tract as well as with the precancerous and cancerous colon but not with normal adult colon. It also reacts with fetal colonic mucosa. Resurgence of gastric mucin reactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fetal colonic) mucins. Cellular Localization: Cytoplasmic.  Negative Species: Bovine.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	> 1,000 kDa
Gene Name	mucin 5AC, oligomeric mucus/gel-forming
Database Link	Entrez Gene 4586 Human UniProt ID P98088
Synonyms	MUC5AC, MUC5, TBM, LeB
Reference Data

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