Mucin 5AC (MUC5AC) Mouse Monoclonal Antibody [Clone ID: SPM297]

CAT#: AM50143PU-T

Mucin 5AC (MUC5AC) mouse monoclonal antibody, clone SPM297, Purified

Size: 20 ug 100 ug



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CNY 3,223.00


货期*
5周

规格
    • 20 ug

Product images

Specifications

Product Data
Clone Name SPM297
Applications FC, IF, IHC, IP, WB
Recommend Dilution ELISA: Use BSA free Antibody for coating.
Flow Cytometry: 0.5-1 μg/million cells.
Immunofluorescence: 1-2 μg/ml.
Western Blotting: 0.5-1 μg/ml.
Immunoprecipitation: 1-2 μg/500 μg protein lysate.
Immunohistochemistry on Frozen and Formalin-fixed Sections: 0.5-1.0 μg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Positive Control: Stomach.
Reactivity Chicken, Feline, Human, Monkey, Mouse, Porcine, Rabbit, Rat
Host Mouse
Clonality Monoclonal
Immunogen M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient.
Specificity This MAb recognizes the peptide core of gastric mucin M1 (>1,000kDa) (recently identified as Mucin 5AC). Its epitope is destroyed by beta-mercaptoethanol and proteases but not by periodate treatment. Antibody to gastric mucin M1 reacts with the gastric epithelium of normal human gastrointestinal tract as well as with the precancerous and cancerous colon but not with normal adult colon. It also reacts with fetal colonic mucosa. Resurgence of gastric mucin reactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fetal colonic) mucins.
Cellular Localization: Cytoplasmic.
Negative Species: Bovine.
Formulation 10mM PBS
State: Purified
State: Liquid purified IgG fraction from Bioreactor Concentrate
Stabilizer: 0.05% BSA
Preservative: 0.05% Sodium Azide
Concentration lot specific
Purification Protein A/G Chromatography
Conjugation Unconjugated
Storage Condition Store undiluted at 2-8°C.
Predicted Protein Size > 1,000 kDa
Gene Name mucin 5AC, oligomeric mucus/gel-forming
Synonyms MUC5AC, MUC5, TBM, LeB
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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