Vimentin (VIM) Mouse Monoclonal Antibody [Clone ID: SPM576]

Specifications

Product Data
Clone Name	SPM576
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	ELISA: Use BSA free Antibody for coating. Flow Cytometry: 0.5-1 µg/million cells  Immunofluorescence: 1-2 µg/ml.  Western Blotting: 0.5-1 µg/ml.  Immunoprecipitation: 1-2 µg/500 µg protein lysate.  Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: Jurkat cells, Sarcomas, Melanomas.
Reactivity	Bovine, Canine, Chicken, Feline, Goat, Human, Porcine
Host	Mouse
Clonality	Monoclonal
Immunogen	Human vimentin recombinant protein.
Specificity	This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP’s) such as Desmin, Keratin, neurofilament, and glial fibrillary acid protein. Anti-Vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent. Cellular Localization: Cytoplasmic.  Negative Species: Mouse and Rat.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	57-60 kDa
Gene Name	vimentin
Database Link	Entrez Gene 7431 Human UniProt ID P08670
Synonyms	VIM
Reference Data

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