CD31 (PECAM1) Mouse Monoclonal Antibody [Clone ID: C31.3 + JC/70A]

Specifications

Product Data
Clone Name	C31.3 + JC/70A
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	Western blot: 0.5-3 µg/ml. Immunoprecipitation: 0.5-1 µg/500 µg protein lysate. Flow Cytometry: 0.5-1 µg/106 cells. Immunofluorescence: 0.5-1 µg/ml. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 min. Positive Control: Tonsil, Angiosarcoma.
Reactivity	Human, Monkey, Rabbit
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant CD31 protein (C31.3) & Membrane preparation of a spleen from a patient with hairy cell leukemia (JC/70A).
Specificity	CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 Monoclonal Antibody reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence. Cellular Localization: Cell surface and cytoplasm of endothelial cells.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	~100 kDa (endothelium) and ~130 kDa (platelets)
Gene Name	platelet and endothelial cell adhesion molecule 1
Database Link	Entrez Gene 5175 Human UniProt ID P16284
Background	CD31 (PECAM-1, or platelet endothelial cell adhesion molecule-1) is a surface protein expressed by endothelial cells, monocytes, platelets, granulocytes, and lymphocyte subsets, and makes up a large portion of endothelial intercellular junctions. CD31 is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. Reports indicate that CD31 interacts with CD38 and is involved in cellular interactions resulting in wound healing and angiogenesis. Expression of CD31 on CD4+ T lymphocytes, helps to control T lymphocyte activation, because in the absence of CD31, T cells have a greater propensity to become activated, resulting in increased susceptibility to become apoptotic. This impact of CD31 loss becomes most pronounced during severe, inflammatory, and immunological stresses such as those caused by systemic Salmonella infection. This identifies a novel role for CD31 in regulating CD4 T homeostasis.
Synonyms	PECAM-1, EndoCAM, GPIIA'
Reference Data

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