EPX Mouse Monoclonal Antibody [Clone ID: AHE-1]

Specifications

Product Data
Clone Name	AHE-1
Applications	IF, IHC
Recommend Dilution	ELISA: For coating, order Ab without BSA. Flow Cytometry: 0.5-1 µg/million cells.  Immunofluorescence: 0.5-1 µg/ml. Immunohistochemistry on Frozen Sections: 0.5-1.0 µg/ml for 30 minutes at RT. Positive Control: MCF-7 cells, Tonsil.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human Eosinophils from a patient with hypereosinophilic syndrome.
Specificity	This Monoclonal Antibody stains eosinophils only and does not stain other peripheral blood cells, including platelets, neutrophils, monocytes, lymphocytes or red blood cells. Human EPX gene product can form a tetramer of two light chains and two heavy chains. Other peroxidase family members include myeloperoxidase (MPO), lactoperoxidase (LPO), and thyroid peroxidase (TPO).  AHE-1 recognizes Human EPX, a granule protein specific to eosinophils. It does not cross-react with eosinophil major basic protein, elastase, cathepsin G, esterase N, thrombin, plasmin, kallikrein, lactoferrin, or transferrin. Cellular Localization: Cytoplasmic, granular.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	70 kDa (55 kDa-Heavy Chain; 15 kDa-Light Chain)
Gene Name	eosinophil peroxidase
Database Link	Entrez Gene 8288 Human UniProt ID P11678
Background	Peripheral blood granulocytes are classified into neutrophils, basophils and eosinophils according to the staining characteristics of their cytoplasmic granules. Granule proteins are released by physiologic and pharmacologic stimuli and play important roles in both normal and pathological host immune responses. Eosinophil major basic protein and eosinophil peroxidase (EPX) are granule proteins specific to the eosinophil.
Synonyms	EPX, EPER, EPO, EPP
Reference Data

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