CCN5 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Indirect ELISA: To detect hCTGFL/WISP-2 by indirect ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. In conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hCTGFL/WISP-2. Sandwich ELISA: To detect hCTGFL/WISP-2 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. In conjunction with Biotinylated Anti-Human CTGFL/WISP-2 as a detection antibody, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hCTGFL/WISP-2. Western Blot: To detect hCTGFL/WISP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCTGFL/WISP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (>98%), 24.3 kDa recombinant human CTGFL/WISP-2
Specificity	This antibody detetcs Connective Tissue Growth Factor-Like protein.
Formulation	PBS, pH 7.2 State: Aff - Purified State: Sterile filtered lyophilized Ig fraction
Reconstitution Method	Restore in sterile water to a concentration of 0.1 - 1.0 mg/ml.
Purification	Immunoaffinity chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	WNT1 inducible signaling pathway protein 2
Database Link	Entrez Gene 8839 Human UniProt ID O76076
Synonyms	WISP-2, CCN5, CT58, CTGFL, CTGF-L, UNQ228/PRO261
Note	Centrifuge vial prior to opening.
Reference Data

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