Apolipoprotein E (APOE) (Isoform E3) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Direct ELISA: To detect Human ApoE3 by Direct ELISA (using 100 µl/well antibody solution) a concentration of ~1.0 µg/ml of this antibody is required. This Biotinylated polyclonal antibody allows the detection of at least 0.2-0.4 ng/well of recombinant Human ApoE3. Sandwich ELISA: To detect Human ApoE3 by Sandwich ELISA (using 100 µl/well) a concentration of 0.25-1.0 µg/ml of this antibody is required. This Biotinylated antibody, in conjunction with Affinity purified anti-Human ApoE3 (AP01142PU-N or AP01142PU-S) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human ApoE3. Western blot: To detect Human ApoE3 this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human ApoE3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	E.Coli derived recombinant Human ApoE3.
Specificity	This antibody detects ApoE3. Cross reacts with Human ApoE2 and Human ApoE4 in both Sandwich ELISA and Western Blot.
Formulation	PBS, pH 7.2 without preservatives. Label: Biotin State: Lyophilized (sterile filtered) purified Ig fraction.
Reconstitution Method	Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography.
Conjugation	Biotin
Storage Condition	Store the lyophilized antibody at -20°C. Following reconstitution it is stable for two weeks at 2-8°C. Frozen aliquots are stable for 6 months when stored at -20°C. Avoid repeated freezing and thawing.
Gene Name	apolipoprotein E
Database Link	Entrez Gene 348 Human UniProt ID P02649
Background	Apolipoprotein E3 mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo E receptor (chylomicron remnant) of hepatic tissues.
Synonyms	ApoE, Apo-E
Note	Centrifuge vial prior to opening. Molecular Weight: ~150 kDa
Reference Data

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