Melanoma Inhibitory Activity (MIA) Rabbit Polyclonal Antibody

CAT#: AP01143PU-N

Melanoma Inhibitory Activity (MIA) rabbit polyclonal antibody, Aff - Purified

Size: 50 ug 100 ug

Conjugation: Unconjugated Biotin



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CNY 4,790.00


货期*
5周

规格
    • 100 ug

Product images

Specifications

Product Data
Applications ELISA, IHC, WB
Recommend Dilution Immunohistochemistry: This antibody stained formalin-fixed paraffin-embedded sections of human pancreas infiltrating ductal adenocarcinoma tissue. The recommended concentration is 1.0-2.0 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detecttion system was used with an alcohol-soluble AEC chromogen. Optimal results for these conditions were achieved with heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer enzyme induced antigen retrievalwith proteinase K at room temperature. Optimal conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network which is funded by the National Cancer Institute.
Indirect ELISA: To detect Human MIA (using 100 µl/well antibody solution) a concentration of 0.5-2.0 µg/ml of this antibody is required. In conjunction with compatible secondary reagents, it allows the detection of at least 0.2-0.4 ng/well of recombinant Human MIA.
Sandwich ELISA: To detect Human MIA (using 100 µl/well antibody solution) a concentration of 0.5-2.0 µg/ml of this antibody is required. In conjunction with Biotinylated Anti-Human MIA (AP01143BT-S or AP01143BT-N) as a Detection antibody, it allows the detection of at least 0.2-0.4 ng/well of recombinant Human MIA.
Western blot: To detect Human MIA this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human MIA is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity Human
Host Rabbit
Clonality Polyclonal
Immunogen Highly pure (> 98%) recombinant Human MIA
Specificity This antibody detects MIA.
Formulation PBS, pH 7.2 without preservatives.
State: Aff - Purified
State: Lyophilized (sterile filtered) Ig fraction.
Reconstitution Method Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification Immunoaffinity Chromatography.
Conjugation Unconjugated
Storage Condition Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Gene Name melanoma inhibitory activity
Background Melanoma Inhibitory Activity (MIA) was originally identified as an inhibitor of the in vitro growth of malignant melanoma cells. It was the first discovered member of a family of secreted cytokines termed the MIA/OTOR family. The four known members of this family; Melanoma Inhibitory Activity, MIA2, OTOR and TANGO each contain a Src homology-3 (SH3)-like domain. Melanoma Inhibitory Activity is an autocrine growth regulatory protein secreted from chondrocytes and malignant melanoma cells that promotes melanoma metastasis by binding competitively to fibronectin and laminin in a manner that results in melanoma cell detachment from the extracellular matrix in vivo. Elevated levels of Melanoma Inhibitory Activity may represent a clinically useful marker for diagnosis of melanoma metastasis as well as a potential marker for rheumatoid arthritis.
Synonyms Melanoma inhibitory activity
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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