Melanoma Inhibitory Activity (MIA) Rabbit Polyclonal Antibody

CAT#: AP01143BT-N

Melanoma Inhibitory Activity (MIA) rabbit polyclonal antibody, Biotin

Size: 25 ug 50 ug

Conjugation: Unconjugated Biotin



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CNY 4,790.00


货期*
5周

规格
    • 50 ug

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Specifications

Product Data
Applications ELISA, WB
Recommend Dilution Direct ELISA: To detect Human MIA (using 100 μl/well antibody solution) a concentration of ~1.0 μg/ml of this antibody is required. It allows the detection of at least 0.2-0.4 ng/well of recombinant Human MIA.
Sandwich ELISA: To detect Human MIA (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. In conjunction with Polyclonal Anti-Human MIA (AP01143PU-S or AP01143PU-N) as a Capture antibody, it allows the detection of at least 0.2-0.4 ng/well of recombinant Human MIA.
Western blot: To detect Human MIA by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human MIA is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity Human
Host Rabbit
Clonality Polyclonal
Immunogen Highly pure (> 98%) recombinant Human MIA
Specificity This antibody detects MIA.
Formulation PBS, pH 7.2 without preservatives.
Label: Biotin
State: Lyophilized (sterile filtered) purified Ig fraction
Reconstitution Method Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1 - 1.0 mg/ml.
Purification Affinity Chromatography.
Conjugation Biotin
Storage Condition Store the lyophilized antibody at -20°C.
Following reconstitution it is stable for two weeks at
2-8°C. Frozen aliquots are stable for 6 months when stored at -20°C.
Avoid repeated freezing and thawing.
Gene Name melanoma inhibitory activity
Background Melanoma Inhibitory Activity (MIA) was originally identified as an inhibitor of the in vitro growth of malignant melanoma cells. It was the first discovered member of a family of secreted cytokines termed the MIA/OTOR family. The four known members of this family; Melanoma Inhibitory Activity, MIA2, OTOR and TANGO each contain a Src homology-3 (SH3)-like domain. Melanoma Inhibitory Activity is an autocrine growth regulatory protein secreted from chondrocytes and malignant melanoma cells that promotes melanoma metastasis by binding competitively to fibronectin and laminin in a manner that results in melanoma cell detachment from the extracellular matrix in vivo. Elevated levels of Melanoma Inhibitory Activity may represent a clinically useful marker for diagnosis of melanoma metastasis as well as a potential marker for rheumatoid arthritis.
Synonyms Melanoma inhibitory activity
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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