beta Defensin 1 (DEFB1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Direct ELISA: To detect Human BD-1 (using 100 µl/well antibody solution) a concentration of ~1.0 µg/ml of this antibody is required. In conjunction with compatible secondary reagents, it allows the detection of at least 0.2-0.4 ng/well of recombinant Human BD-1. Sandwich ELISA: To detect Human BD-1 (using 100 µl/well antibody solution) a concentration of 0.25-1.0 µg/ml of this antibody is required. In conjunction with Polyclonal Anti-Human BD-1 (Cat.-No AP01151PU-N or AP01151PU-S) as a capture antibody, it allows the detection of at least 0.2-0.4 ng/well of recombinant Human BD-1. Western blot: To detect Human BD-1 this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human BD-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	E. Coli derived recombinant Human beta Defensin-1
Specificity	This antibody detects Human BD-1.  Other species not tested.
Formulation	PBS, pH 7.2 without preservatives. Label: Biotin State: Lyophilized (sterile filtered) purified Ig fraction
Reconstitution Method	Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography
Conjugation	Biotin
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month  or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	defensin beta 1
Database Link	Entrez Gene 1672 Human UniProt ID P60022
Background	Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 1, an antimicrobial peptide implicated in the resistance of epithelial surfaces to microbial colonization. This gene maps in close proximity to defensin family member, defensin, alpha 1 and has been implicated in the pathogenesis of cystic fibrosis. The mature form of Beta defensin 1 is 36 amino acids.
Synonyms	Beta-defensin 1, BD1, BD-1, HBD1, hBD-1, DEFB1, DEFB101
Reference Data

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