beta Defensin 1 (DEFB1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Indirect ELISA: To detect hBD-1 (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This In conjunction with compatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBD-1. Sandwich ELISA: To detect hBD-1 (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. In conjunction with Biotinylated Anti-Human BD-1 as a detection antibody, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBD-1. Western blot: To detect hBD-1 this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBD-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (> 98%) E.coli derived 5.0 kDa recombinant human BD-1
Specificity	This antibody detects Defensin beta 1.
Formulation	PBS, pH 7.2 without preservatives. State: Aff - Purified State: Lyophilized (sterile filtered) purified Ig fraction
Reconstitution Method	Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1 - 1.0 mg/ml.
Purification	Immunoaffinity chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	defensin beta 1
Database Link	Entrez Gene 1672 Human UniProt ID P60022
Background	Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 1, an antimicrobial peptide implicated in the resistance of epithelial surfaces to microbial colonization. This gene maps in close proximity to defensin family member, defensin, alpha 1 and has been implicated in the pathogenesis of cystic fibrosis. The mature form of Beta defensin 1 is 36 amino acids.
Synonyms	Beta-defensin 1, BD1, BD-1, HBD1, hBD-1, DEFB1, DEFB101
Reference Data

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