IKB alpha (NFKBIA) pSer32/36 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western Blot: 1/500-1/1000. Immunohistochemistry on paraffin sections: 1/50-1/200. Immunofluorescence: 1/50-1/200.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Specificity	This antibody detects endogenous levels of IkB-alpha pSer32/36 protein.
Formulation	Phosphate buffered saline (PBS), pH~7.2 containing 0.05% Sodium Azide as preservative. State: Aff - Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
Concentration	1.0 mg/ml
Purification	Affinity Chromatography using epitope-specific immunogen.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	~ 36 kDa
Gene Name	NFKB inhibitor alpha
Database Link	Entrez Gene 4792 Human UniProt ID P25963
Background	On the basis of both functional and structural considerations, members of the IκB family of proteins can be divided into four groups. The first of these groups, IκB-α (a 35-37 kDa protein), includes the avian protein pp40 and the mammalian MAD-3, both of which inhibit binding of p50-p65 NFκB complex or Rel protein to their cognate binding sites but do not inhibit the binding of p50 homodimer to κB sites, suggesting that the IκB-α family binds to the p65 subunit of p50-p65 heterocomplex through Ankyrin repeats. The second member of the IκB family is represented by a 45 kDa protein designated IκB-β. The third group of IκB proteins is represented by IκB-γ, a 70 kDa protein identical in sequence with the C-terminal domain of the p110 precursor of NFκB p50 and expressed predominantly in lymphoid cells. An additional IκB family member has been identified as IκB-ε, a 45 kDa protein which has several phosphorylated forms and is primarily found complexed with RelA and/or c-Rel.
Synonyms	I-kappa-B-alpha, MAD3, NFKBI, I kappa B-alpha, IkappaBalpha, IkB-alpha
Reference Data

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