c-Jun (JUN) pThr239 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western blot: 1/500 - 1/1000; Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight. Immunohistochemistry on paraffin sections: 1/50 - 1/100. Immunofluorescence: 1/100 - 1/200.
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic phosphopeptide derived from human c-Jun around the phosphorylation site of threonine 239 (G-E-TP-P-P).
Specificity	c-Jun (phospho-Thr239) antibody detects endogenous levels of c-Jun only when phosphorylated at threonine 239.
Formulation	Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl containing 0.02% Sodium Azide as preservative and 50% glycerol as stabilizer State: Aff - Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Immunoaffinity chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	Jun proto-oncogene, AP-1 transcription factor subunit
Database Link	Entrez Gene 3725 Human UniProt ID P05412
Background	The human protooncogene JUN is the putative transforming gene of avian sarcoma virus 17, and it encodes a protein which is highly homologous to the viral protein. cJun (previously known as the Fos binding protein p39) and c Fos form a complex in the nucleus. AP 1 (activating protein 1) is a collective term referring to these dimeric transcription factors composed of Jun, Fos or ATF subunits that bind to a common DNA site, the AP1 binding site. AP 1 proteins, mostly the Jun group, regulate the expression and function of cell cycle regulators such as Cyclin D1, p53, p21 (cip1/waf1), p19 (ARF) and p16. Fos and Jun proto oncogene expression is induced transiently by a variety of extracellular stimuli associated with mitogenesis, differentiation processes or depolarization of neurons. JUN has been mapped to 1p32 to p31, a chromosomal region involved in both translocations and deletions in human malignancies.
Synonyms	Transcription factor AP1
Reference Data

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