BCR pTyr177 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Suitable for use in Western blot (1/500-1/1000) and Immunohistochemistry on Paraffin-Embedded Sections (1/50-1/100).
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	The antiserum was produced against synthesized phosphopeptide derived from Human Bcr around the phosphorylation site of Tyrosine 177 (P-F-Yp-V-N).
Specificity	This antibody detects endogenous levels of Bcr only when phosphorylated at Tyrosine 177.
Formulation	PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol State: Aff - Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Purification	Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Conjugation	Unconjugated
Storage Condition	Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	BCR, RhoGEF and GTPase activating protein
Database Link	Entrez Gene 613 Human UniProt ID P11274
Background	The breakpoint cluster region protein (Bcr) is best know to be involved in genomic translocation with fusion partner Abl (Cbr-Abl) causing chronic myelogenous leukemia (CML). This 160 kDa protein contains a serine/threonine kinase domain, an SH2 binding domain, a GTP/GDP exchange domain and a C-term domain which functions as a GTPase activating protein for p21rac and CDC42. Additionally, Bcr is involved in signal transduction and can down regulate Ras mediated cell signaling.
Synonyms	Breakpoint cluster region protein, BCR1, D22S11, NY-REN-26
Note	Molecular Weight: 210 kDa
Reference Data

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