NFkB p100 / p52 (NFKB2) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western Blot: 1/500~1/1000. Immunohistochemistry: 1/50~1/100.
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	This antiserum was produced against synthesized non-phosphopeptide derived from human NFkB-p100/p52 around aa 868-872 (Y-G-S-Q-S).
Specificity	This antibody (AP02559PU) detects endogenous levels of total NF-kB p100/p52 protein.
Formulation	PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide as preservative and 50% Glycerol as stabilizer. State: Aff - Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Purification	Immunoaffinity Chromatography using epitope-specific immunogen.
Conjugation	Unconjugated
Storage Condition	Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	nuclear factor kappa B subunit 2
Database Link	Entrez Gene 4791 Human UniProt ID Q00653
Background	NFkB is formed through the association of multiple subunits, either as a homodimer or heterodimer. Subunits have been identified as p50 (NFkB1), p65 (RelA), c-Rel, RelB and p52 (NFkB2). The classic NFkB form exists as a p50-p65 heterodimer and predominates in many cell types. Many of the possible combinatorial forms of homo- and heterodimers have been identified and growing evidence indicates that different forms of NFkB have different functions in cells. Interestingly, both the p50 and p52 subunits are derived from the precursor proteins p105 and p100 respectively, that each contain multiple copies of the so called ankyrin repeat at their C termini. Nuclear translocation of NFkB is confirmed by the use of electrophorectic mobility shift assays or by immunoblotting with nuclear extracts. The subunit composition of NFkB is confirmed by the use of antibodies that "supershift" the DNA/protein complex.
Synonyms	LYT10, KBF2, H2TF1, NF-kB p100, NF-kB p52, NF kappa B
Reference Data

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