FOXO1 (254-258) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western blot: 1/500-1/1000. Incubate membrane with diluted antibody in 5% nonfat milk, 1x TBS, 0.1%Tween-20 at 4°C with gentle shaking, obernight. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin sections: 1/50-1/100.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide Sequence around amino acids 254~258 (A-A-S-M-D) derived from Human FKHR/FOXO1A
Specificity	This antibody detects endogenous levels of total FKHR protein.
Formulation	PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol. State: Aff - Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Purification	Immunoaffinity Chromatography using epitope-specific immunogen.
Conjugation	Unconjugated
Storage Condition	Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Predicted Protein Size	78-82 KDa
Gene Name	forkhead box O1
Database Link	Entrez Gene 2308 Human UniProt ID Q12778
Background	FOXO1A (FKHR or ForkHead in Rhabdomyosarcoma) is a 70 kDa protein which is a member of a subfamily of the forkhead homeotic gene family of transcription factors. Recent studies have shown that this protein can act as either a coactivator or a corepressor of nuclear receptor activity. This action is mediated through the LXXLL motif found in the C terminus of the FOXO1A protein. The specific function of the gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Chromosomal aberrations involving FOXO1A are a cause of rhabdomyosarcoma 2 (RMS2); also known as alveolar rhabdomyosarcoma.
Synonyms	Forkhead Box Protein O1, FOXO1, FKHR
Reference Data

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