IKB alpha (NFKBIA) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Suitable for use in in Western blot (1/500-1/1000) and Immunohistochemistry (1/50-1/100).
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	The antiserum was produced against synthesized non-phosphopeptide derived from human IκB-α around the phosphorylation site of tyrosine 42 (E-E-Yp-E-Q).
Specificity	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. IκB-α antibody AP02694PU detects endogenous levels of total IκB-α protein.
Formulation	PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol State: Aff - Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Immunoaffinity chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	NFKB inhibitor alpha
Database Link	Entrez Gene 4792 Human UniProt ID P25963
Background	Three major forms of IKB like molecules have been identified and each is characterised by multiple copies of ankyrin repeats. IKB alpha and IKB beta appear to be the major regulatory forms of IKB in most cells. These proteins interact with p65 or cRel containing forms of NFkB and block nuclear import by masking the nuclear localisation sequences of NFkB. The activation of NFkB involves the inducible phosphorylation and subsequent degradation of IKB. Immunoblotting easily detects the hyperphosphorylated forms of IKB alpha, but not phosphorylated IKB beta. Interestingly, IKB alpha and IKB beta mediate different NFkB responses. IkB alpha appears to control more transient activation of NFkB in response to an inducer, while IKB beta controls a persistent response. Bcl3 interacts with p50 and p52 containing forms of NFkB, but rather than being an inhibitor it appears to function to stimulate transcription. The degradation of IKB is confirmed by immunoblotting.
Synonyms	I-kappa-B-alpha, MAD3, NFKBI, I kappa B-alpha, IkappaBalpha, IkB-alpha
Reference Data

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