Notum Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western blot: 5-10 µg/ml. Immunohistochemistry.
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant full-length notum protein from Mouse
Specificity	This antibody detects Notum.
Formulation	Phosphate buffered saline with 0.08% Sodium Azide as preservative State: Purified State: Liquid purified Ig fraction
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the product (in aliquots) at -20°C. Can be shipped at 2-8°C. Avoid repeated freezing and thawing.
Gene Name	notum pectinacetylesterase homolog (Drosophila)
Database Link	Entrez Gene 77583 Mouse UniProt ID Q8R116
Background	Genetic studies in Drosophila have revealed a novel secreted antagonist for Wingless (Wg) signaling, referred to as Notum/Wingful (Wf). Wf/Notum encodes a protein of 430 amino acid residues, which is related to plant pectin acetylesterases of the alpha/beta-hydrolase superfamily. The main target of Notum action appears to be cell surface proteoglycans Dally and/or Dally-like (Dlp). Notum governs the extracellular Wg morphogen distribution via Dally and Dlp. Also, like some vertebrate Wnt antagonists, notum expression is activated by Wg signaling, revealing the existence of a feedback control mechanism in which Wg modifies its own gradient distribution. In vertebrate, proteoglycans related to Dally and Dlp likely play important roles in Wnt signaling which encodes a glypican that is required for Wnt-11 signaling. A single ortholog of Notum exists in the mouse and the human genome, implying that a similar regulatory mechanism may exist in vertebrates.
Synonyms	NOTUM
Reference Data

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