Collagen VI (COL6A1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC, IP, WB
Recommend Dilution	ELISA. Immunofluorescence. Immunohistochemistry on Paraffin Sections: 2.5 µg/ml. Immunoprecipitation. Western Blot.
Reactivity	Bovine, Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Collagen Type VI from Human and Bovine placenta
Specificity	This antibody detects Collagen, Type VI from Human and Bovine Placenta. Other species not tested.
Formulation	0.02 M Potassium Phosphate, pH 7.2, 0.15 M Sodium Chloride with 0.01% (w/v) Sodium Azide as preservative State: Aff - Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Immunoaffinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	collagen type VI alpha 1
Database Link	Entrez Gene 1291 Human UniProt ID P12109
Background	Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Collagens are extensively purified for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used.
Synonyms	COL6A1
Reference Data

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