c Abl (ABL1) pTyr393/439 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Immunofluorescence: 1/100 - 1/200. Immunohistochemistry on Paraffin-Embedded Sections: 1/50 - 1/100. Western Blot: 1/500 - 1/1000; Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight.
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic phosphopeptide derived from human ABL1/2 around the phosphorylation site of Tyrosine 393/439 (D-T-Yp-T-A).
Specificity	ABL1/ABL2 antibody detects endogenous levels of ABL1/2 only when phosphorylated at Tyrosine 393/439.
Formulation	PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol. State: Aff - Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	ABL proto-oncogene 1, non-receptor tyrosine kinase
Database Link	Entrez Gene 25 Human UniProt ID P00519
Background	ABL1, an Abl type protein kinase, is associated with cell differentiation, cell division, cell adhesion and stress response. Kinase activity of nuclear ABL1 is mediated by retinoblastoma protein. Additionally, ABL1 has been shown to bind nuclear DNA, and this binding activity is regulated by CDC2-mediated phosphorylation. ABL1 (-/-) mice are osteoporotic and display increased perinatal mortality, reduced fertility, foreshortened crania and defects in the maturation of B cells in bone marrow. At least two mRNA isoforms have been reported, 6- or 7-kb, with alternatively spliced first exons joined to the common exons 2-11. Alterations of ABL1 by chromosomal rearrangement or viral transduction lead to malignant transformation. Further, a very long intron in the ABL1 gene is a target for translocations. Translocations of ABL1 to the breakpoint cluster region (BCR) on chromosome 22 lead to chronic myeloid leukemia and acute lymphocytic leukemia.
Synonyms	c-ABL, p150, ABL1, ABL2, JTK7, bcr/abl
Reference Data

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