CBL pTyr700 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Immunofluorescence: 1/100 - 1/200. Immunohistochemistry on Paraffin-Embedded Sections: 1/50 - 1/100. Western Blot: 1/500 - 1/1000; Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic phosphopeptide derived from human c-Cbl around the phosphorylation site of Tyrosine 770 (T-E-YP-M-T).
Specificity	This Antibody detects endogenous levels of c-Cbl only when phosphorylated at Tyrosine 770.
Formulation	PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol. State: Aff - Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	Cbl proto-oncogene
Database Link	Entrez Gene 867 Human UniProt ID P22681
Background	The proto-oncogene c-CBL was initially identified as the cellular homologue of v-CBL oncogene that induces pre-B cell lymphomas and myeloid leukemias in mice. In more recent studies CBL has been shown to be a negative regulator of tyrosine kinase signaling. The ubiquitin ligase activity of CBL leads to the degradation of tyrosine kinases, thus attenuating the signal of receptors. Targets of CBL include activated protein tyrosine kinases belonging to the Src and Syk/Zap-70 families. An additional mechanism to attenuate receptor signaling is thought to be achieved by CBL’s interaction with downstream targets of tyrosine kinases, such as PI-3K and Vav.
Synonyms	RING finger protein 55, CBL2, c-CBL
Reference Data

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