Cofilin 1 (CFL1) (+ Cofilin 2) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western Blot: 1/500 - 1/1000. Immunofluorescence: 1/100 - 1/200. Immunohistochemistry on Paraffin-Embedded Sections: 1/50 - 1/100.
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	The antiserum was produced against synthesized non-phosphopeptide derived from human Cofilin1/Cofilin2 around the phosphorylation site of Tyrosine 86~90(A-T-Y-E-T).
Specificity	This antibody detects endogenous levels of total Cofilin-1/2 protein.
Formulation	PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol. State: Aff - Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Purification	Immunoaffinity Chromatography.
Conjugation	Unconjugated
Storage Condition	Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	cofilin 1
Database Link	Entrez Gene 12631 MouseEntrez Gene 1072 Human UniProt ID P23528
Background	Cofilin is ubiquitously expressed in eukaryotic cells where it binds to Actin, thereby regulating the rapid cycling of Actin assembly and disassembly essential for cellular viability. Cofilin is a low molecular weight protein that binds to filamentous F-Actin by bridging two longitudinally associated Actin subunits changing the F-Actin filament twist. This process is allowed by the dephosphorylation of Cofilin Ser 3 by factors such as opsonized zymosan. Lim kinase 1, a serine kinase, phosphorylates Cofilin and renders it unable to bind and depolymerise F-Actin.
Synonyms	CFL1, CFL, p18
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Axon guidance, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.