S100A1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC
Recommend Dilution	Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections and cell smears): 1/50-1/200 using streptavidin~biotin system or polymer system. Immunofluorescence. This antibody should not be diluted in buffer which contains detergents. Positive control: Melanoma or schwannoma.
Reactivity	Bovine, Human, Monkey, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Purified S100 protein from Bovine.
Specificity	This antibody stains schwannoma, ependymomas, astrogliomas, almost all benign and malignant melanoma and their metasis. Cellular localization: Cytoplasmic.
Formulation	10 mM PO4, 150 mM NaCl, pH 7.4 containing 1% BSA and 0.05% Sodium Azide as preservative. State: Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Purification	Protein A Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Gene Name	S100 calcium binding protein A1
Database Link	Entrez Gene 20193 MouseEntrez Gene 295214 RatEntrez Gene 6271 Human UniProt ID P23297
Background	S100 protein is a type of low molecular weight protein found in vertebrates characterized by two calcium binding sites of the helix-loop-helix ("EF-hand type") conformation. There are at least 21 different types of S100 proteins. The name is derived from the fact that the protein is 100% Soluble in ammonium sulfate at neutral pH. Most S100 proteins are homodimeric, consisting of two identical polypeptides held together by non-covalent bonds. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. Although S100 proteins are structurally similar to calmodulin, they differ in that they are cell-specific, expressed in particular cells at different levels depending on environmental factors. To contrast, calmodulin is a ubiquitous and universal intracellular Ca++ receptor widely expressed in many cells.S-100 is normally present in cells derived from the neural crest (Schwann cells, melanocytes, and glia cells), chondrocyte, adipocytes, myoepithelial cells, macrophages, Langerhans cells, dendritic cells, and keratinocytes. It may be present in some breast epithelial cells.
Synonyms	S100 Calcium binding protein, S-100 protein, S100A, S100B, S-100 protein alpha chain, S-100 protein beta chain, Astrocyte Marker
Reference Data

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