Luciferase (Photinus pyralis) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Western blot : 1/1,000 - 1/5,000. ELISA : 1/10,000 - 1/25,000. Firefly-luciferase produces a green light with a wavelength of 562 nm; Expect a band at approximately 60.7 kDa in size corresponding to Luciferase by western blotting in the appropriate cell lysate or extract. Anti-Luciferase has been assayed against 1.0 µg of Luciferase in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature.  A working dilution of 1/80,000 to 1/310,000 of the reconstitution concentration is suggested.
Host	Goat
Clonality	Polyclonal
Immunogen	Luciferase from Photinus pyralis (Firefly)
Specificity	This antibody reacts to Luciferase. Immunoelectrophoresis give a single precipitin arc against anti-biotin, anti-goat serum as well as purified and partially purified Luciferase [Photinus pyralis (Firefly)].  No reactivity is observed against Sea pansy (Renilla reniformis) luciferase.
Formulation	0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 Label: Biotin State: Purified State: Lyophilized IgG fraction Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free) Preservative: 0.01% (w/v) Sodium azide
Reconstitution Method	Restore with 0.1 ml of deionized water (or equivalent). For extended storage, mix product with glycerol to 50% final.
Concentration	lot specific
Purification	Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation	Biotin
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	UniProt ID P08659
Background	Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
Reference Data

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