Luciferase (Photinus pyralis) Goat Polyclonal Antibody

Specifications

Product Data
Applications	FC, IF
Recommend Dilution	Immunofluorescence: 1/500 - 1/2500.
Host	Goat
Clonality	Polyclonal
Immunogen	Luciferase (Photinus pyralis (Firefly))
Specificity	This antibody reacts to Luciferase.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml Bovine Serum Albumin (BSA) as stabilizer and 0.01% (w/v) Sodium Azide as preservative Label: FITC State: Lyophilized IgG fraction Label: Fluorescein isothiocyanate Absorption emission: 495 nm / 528 nm Molar radio: 2.5 moles FITC per mole of Goat IgG
Reconstitution Method	Restore with 2.0 ml of deionized water (or equivalent). For extended storage, mix product with glycerol to 50% final
Concentration	lot specific
Purification	Ion exchange chromatography
Conjugation	FITC
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	390 kDa.
Database Link	UniProt ID P08659
Background	Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
Reference Data

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