SAE1 Rabbit Polyclonal Antibody
CAT#: AP09135PU-N
SAE1 rabbit polyclonal antibody, Purified
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CNY 8,869.00
货期*
4周
规格
Specifications
Product Data | |
Applications | ELISA, IF, IHC, WB |
Recommend Dilution | ELISA: 1/5,000-1/20,000. Western Blot: 1/500 1/2,000. Immunofluorescence. Immunohistochemistry on Paraffin Sections. |
Reactivity | Bovine, Canine, Chimpanzee, Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | Recombinant protein produced by baculoviral expression in insect cells (Sf9, Spodoptera frugiperda) corresponding to full length Human SUMO Activating Enzyme E1 fused with GST |
Specificity | This antibody is directed against SUMO Activating Enzyme E1 protein. |
Formulation | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01% (w/v) Sodium Azide State: Purified State: Lyophilized purified IgG fraction Stabilizer: None |
Reconstitution Method | Restore with 0.1 ml of deionized water or equivalent. |
Concentration | lot specific |
Purification | Affinity Chromatography on Protein A |
Conjugation | Unconjugated |
Storage Condition | Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
Gene Name | SUMO1 activating enzyme subunit 1 |
Database Link | |
Background | SUMO E1 activating enzyme (also called Ubiquitin-like 1 activating enzyme E1A, UBLE1A, AOS1, SAE1, and SUA1) is a heterodimeric (SAE1/SAE2) enzyme that activates the ubiquitin-like SUMO proteins (SUMO stands for Small Ubiquitin-like MOdifier.) The SAE1 (SUMO Activating Enzyme 1, also called Aos1) subunit resembles the Nterminal half of yeast UBA1; the SAE2 (also called Uba2) subunit corresponds to the C-terminal part of yeast UBA1 and contains the active site cysteine. In the SUMO activation step, SAE1/SAE2 uses ATP to adenylate the C-terminal glycine of SUMO-1 (the first of the three different mammalian SUMO proteins) then forms a high-energy thioester bond between the C-terminal glycine and the active site cysteine in SAE2 (Uba2). In the conjugation step, the SUMO moiety is transferred from SAE1/SAE2 to the active site cysteine (Cys 93) of the SUMO conjugating enzyme (SUMO E2, Ubc9) forming a SUMO-E2 thioester complex. |
Synonyms | SUA1, UBLE1A |
Reference Data |
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