FITC Goat Polyclonal Antibody

Specifications

Product Data
Applications	FC
Recommend Dilution	Flow Cytometry. Immunofluorescence.
Host	Goat
Clonality	Polyclonal
Immunogen	Fluorescein conjugated to Keyhole Limpet Hemocyanin (KLH)
Specificity	This antibody reacts to Fluorescein.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml Bovine Serum Albumin (BSA) as stabilizer and 0.01% (w/v) Sodium Azide as preservative Label: TRITC State: Lyophilized purified Ig Label: Tetramethylrhodamine isothiocyanante (Molecular Weight 444 daltons) Absorption emission: 550 nm / 570 nm Molar radio: 2.0 moles TRITC per mole of Goat IgG
Reconstitution Method	Restore with 1,0 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Affinity chromatography
Conjugation	TRITC
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.
Synonyms	Fluorescein Isothiocyanate
Reference Data

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