Transferrin (TF) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. ELISA: 1:3,000 - 1:12,000. Western blot: 1:300 - 1:1,000.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Human Transferrin
Specificity	This antibody is specific for Transferrin (TF).
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free, 0.01% (w/v) Gentamicin Sulfate Label: HRP State: Lyophilized Ig fraction Label: Horseradish Peroxidase
Reconstitution Method	Restore with 0.5 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	This reagent was purified from pooled rabbit sera obtained from rabbits immunized with Human Transferrin. Purification was by Protein A Sepharose Fast Flow followed by POROS EP antigen resin affinity purification. The purified antibody was then conjugated to horseradish peroxidase and subsequently purified followed by exhaustive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, purified Human Transferrin and Human Serum.
Conjugation	HRP
Storage Condition	Store vial at 2-8 ° C prior to restoration. Following restoration product can be stored undiluted at 2-8 ° for up to one month or (in aliquots) at -20 °C or below. For extended storage add glycerol to 50%. Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
Gene Name	transferrin
Database Link	Entrez Gene 7018 Human UniProt ID P02787
Synonyms	Serotransferrin, Siderophilin
Reference Data

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