Amyloid beta (1-14) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC, WB
Recommend Dilution	ELISA: 1/10,000 - 1/50,000. Western blot: 1/1000 - 1/5000. A 40-50 kD band consistent with a higher MW precursor is detected in using whole tissue extracts from mouse brain. Immunofluorescence. 1/50-1/200. Immunohistochemistry: 1/50 - 1/200.  We recommend the use of 4% PFA for Paraffin embedded tissues and 10% Formalin for Frozen tissue for fixation.
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide corresponding to the amino terminus (1-14) of Human beta amyloid conjugated to KLH using maleimide.
Specificity	This antibody is directed against the amino terminal end of beta amyloid and is useful in determining its presence in various assays.
Formulation	0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide as preservative. State: Aff - Purified State: Liquid (sterile filtered) purified Ig fraction
Concentration	lot specific
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or(in aliquots) at -20°C or below for longer . Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Background	Beta amyloid, often abbreviated as A-beta, is a protein that builds up in the brains of persons with Alzheimer's disease, collecting in clumps called plaques or senile plaques. While some researchers question whether beta amyloid is the cause of the dementia, most agree that it is involved in the disruption of thinking that is a hallmark of the disease. In some cases of familial Alzheimer's disease, mutations in genes for the proteins called the presenilins lead to increased production of amyloid. Researchers have been looking at how presenilin-1 in particular contributes to the excess buildup of beta amyloid. Presenilin-1 apparently acts to increase the activity of gamma-secretase, an enzyme that changes a normal protein (amyloid precursor protein or APP) into beta amyloid itself. Furthermore, presenilin-1 might be gamma-secretase.
Reference Data

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