RAD54 (RAD54L) (1-17) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Suitable for use in ELISA and by Western blot. Expect a band approximately 84 kDa in size corresponding to RAD54 protein by Western blotting in the appropriate cell lysate or extract. Splice variants exist for this protein that may result in the detection of lower molecular weight bands. Recommended Dilutions: ELISA: 1/10000-1/40000. Western Blot: 1/500-1/2000.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide corresponding aa 1-17 of Human RAD54 protein.
Specificity	BLAST analysis indicates 100% homology of the immunizing sequence with RAD54 from Human. Cross reactivity with RAD54 protein homologues from other sources may not occur as sequence homology varies by at least one amino acid residue in this sequence.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01% (w/v) Sodium Azide as preservative. State: Aff - Purified State: Liquid (sterile filtered) purified Ig fraction.
Concentration	lot specific
Purification	Immunoaffinity Chromatography using peptide coupled to agarose beads.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	RAD54-like (S. cerevisiae)
Database Link	Entrez Gene 8438 Human UniProt ID Q92698
Background	RAD54, also known as hHR54, HR54, hRAD54 and RAD54A, belongs to the DEAD-like helicase superfamily, and shares similarity with Saccharomyces cerevisiae Rad54, a protein known to be involved in the homologous recombination and repair of DNA. This protein has been shown to play a role in homologous recombination related repair of DNA double-strand breaks. The binding of this protein to double-strand DNA induces a DNA topological change, which is thought to facilitate homologous DNA pairing, and stimulate DNA recombination.
Synonyms	RAD54A, RAD54 homolog, hRAD54, hHR54
Reference Data

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