PARK7 (177-189) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA: 1:10,000 - 1:50,000. Immunohistochemistry on paraffin sections: 2 µg/ml - 5 µg/ml. Western blot: 1:500 - 1:2,000 (Expect a band approximately 28 kDa in size corresponding to PARK7 in the appropriate cell lysate or extract).
Reactivity	Chimpanzee, Human, Monkey, Zebrafish
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide corresponding aa 177-189 of Human PARK7 protein
Specificity	Thisa ntibody is specific for PARK7.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide State: Aff - Purified State: Liquid sterile filtered Ig fraction
Concentration	lot specific
Purification	Immunoaffinity chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Gene Name	Parkinsonism associated deglycase
Database Link	Entrez Gene 11315 Human UniProt ID Q99497
Background	The product of the PARK7 gene, known as PARK7, Parkinson disease (autosomal recessive, early onset) 7, DJ-1 mutant, and DJ-1 oncogene product, functions as a positive regulator of androgen receptor-dependent transcription. PARK7 may also function as a redoxsensitive chaperone and as a sensor for oxidative stress and also has been reported to prevent aggregation of SNCA. PARK7 protects neurons against oxidative stress and cell death and plays a role in fertilization. While PARK7 has no proteolytic activity, it does have a weak transforming activity. PARK7 forms a homodimer and binds to DJBP and PIAS2. PARK7 is part of a ternary complex containing PARK7, DJBP and AR and shows both a nuclear and cytoplasmic localization. In some cells, PARK7 is associated with mitochondria, particularly after oxidative stress. This protein is highly expressed in pancreas, kidney, skeletal muscle, liver, testis and heart tissue, and is expressed at lower levels in placenta and brain. PARK7 is detected in tau inclusions in brains from neurodegenerative disease patients, in astrocytes, Sertoli cells, spermatogonia, spermatids and spermatozoa. Defects in PARK7 are the cause of autosomal recessive early-onset Parkinson's disease 7 (PARK7). Parkinson's disease (PD) is a complex, multifactorial disorder that is characterized by bradykinesia, resting tremor, muscular rigidity and postural instability, response to treatment with levodopa, the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins) in surviving neurons.
Synonyms	Oncogene DJ1, Parkinson disease protein 7
Reference Data

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