CDC27 pSer427 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	ELISA: 1/5,000 - 1/25,000. Western Blot: 1/500 - 1/2,500.
Reactivity	Canine, Chimpanzee, Chicken, Human, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide corresponding aa 422-430 of Human CDC27
Specificity	This antibody is directed against the phosphorylated form of CDC27 at the pS427 residue.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01% (w/v) Sodium Azide State: Aff - Purified State: Liquid purified Ig
Concentration	lot specific
Purification	Affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody at -20°C. Avoid repeated freezing and thawing.
Gene Name	cell division cycle 27
Database Link	Entrez Gene 996 Human UniProt ID P30260
Background	Human CDC27 (also called Cell division cycle protein 27 homolog, CDC27Hs and H-NUC) shares strong similarity with Saccharomyces cerevisiae protein Cdc27. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of a cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis.
Synonyms	CDC27Hs, H-NUC, D0S1430E, D17S978E
Reference Data

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