Biotin (F(ab')2 fragment) Goat Polyclonal Antibody

Specifications

Product Data
Applications	FC, IF
Recommend Dilution	Immunofluorescence. Flow Cytometry.
Host	Goat
Clonality	Polyclonal
Immunogen	Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
Specificity	This is a Fluorescein conjugated F(ab)2 fragment of Anti-Biotin.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml Bovine Serum Albumin (BSA) and 0.01% (w/v) Sodium Azide Label: FITC State: Lyophilized F(ab)2 fragment Label: Fluorescein isothiocyanate (Molecular Weight 390 daltons) Absorption emission: 495 nm / 528 nm Molar radio: 3.6 moles FITC per mole of Goat IgG
Reconstitution Method	Restore with 1.0 ml of deionized water (or equivalent). For extended storage, add glycerol to 50%
Concentration	lot specific
Purification	Immunoaffinity chromatography
Conjugation	FITC
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
Synonyms	Vitamin B7, Vitamin H
Reference Data

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