AHNAK Guinea Pig Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, IP, WB
Recommend Dilution	Western Blot: 1/1,000-1/2,000 (ECL). Immunoprecipitation. Immunofluorescence. Immunohistochemistry on Frozen Sections: 1/50-1/100. Incubation Time: 1 h at RT.
Reactivity	Bovine, Human
Host	Guinea Pig
Clonality	Polyclonal
Immunogen	Synthetic peptides of Human Desmoyokin (D1a/b, D2a/b, D4a/b), coupled to KLH
Specificity	This antibody is specific for Desmoyokin (AHNAK antigen), an approx.600 kD protein localized e.g. in interdesmosomal membrane parts of epithelia and cortical lens fiber cells, but also found in the cytoplasm and nuclei of epithelia.
Formulation	State: Serum State: Liquid Stabilized Antiserum Preservative: 0.09% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Gene Name	AHNAK nucleoprotein
Database Link	Entrez Gene 79026 Human UniProt ID Q09666
Background	AHNAK1 (Desmoyokin) is a large (700 kDa) scaffold protein that translocates to the plasma membrane after an increas of extracellular calcium level or upon proteinkinase C activation and regulates extracellular calcium influx mediated by L-type Ca2+ channels. AHNAK1 has been implicated in diverse signal transduction proceses affecting cell differentiation and proliferation. In response to calcium-dependent intercellular contacts AHNAK1 forms multimeric complexes in the plasma membrane, connected with actin and annexin 2/S100A10 assemblies and is thus involved in organization of the plasma membrane architecture. In epithelial cells, AHNAK1 is localized in cytoplasm or is membrane-associated, but in cells of nonepithelial origin AHNAK1 is predominantly nuclear; it has a weak DNA-binding activity and associates with the DNA-ligase IV-XRCC4 complex.
Synonyms	Desmoyokin
Reference Data
Protein Families	Protease

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